Profile image
By Alton Parrish (Reporter)
Contributor profile | More stories
Story Views

Last Hour:
Last 24 Hours:

Halting Mosquitoes on a Continental Scale

Monday, November 21, 2016 19:55
% of readers think this story is Fact. Add your two cents.
Researchers have solved key problems involving using gene drive technology to stop spread of disease-carrying mosquitoes.

A team of researchers, including two from the University of California, Riverside, have made a key discovery that could potentially help eliminate disease-spreading mosquitoes on a continent-wide scale.

The research, just published in the preprint server for biology, bioRxiv, focuses on the stability of so-called gene drive systems, which involve adding, disrupting, or modifying genes to alter or suppress a population of an organism.

Gene drives have been proposed as an inexpensive, environmentally friendly, and long-lasting way to address significant ecological and public health-related problems, including mosquito-borne diseases such as malaria, Zika virus, dengue fever, yellow fever, and others.

Photo Credit: James Gathany, Centers For Disease Control And Prevention’s Public Health Image Library
Although gene drives have been discussed and studied for multiple decades, the CRISPR/Cas9 gene editing technique which relies on a Cas9 protein targeted to a specific genomic location by guide RNAs has recently revolutionized the development of gene drive systems because it offers an inexpensive, efficient, and reliable way to make precise, targeted changes to the genome.

There are different types of gene drives that vary in their inheritance mechanisms, including a type known as homing-based gene drive, which can be used to suppress populations. Past studies have found that the homing-based method can result in 90 to 99 percent of offspring inheriting the altered gene structure, as opposed to the 50 percent expected with traditional genetics, making this system extraordinarily powerful.

Although the high inheritance rate seen with these homing systems is quite promising, the small percentage of offspring that don’t inherit the genetic change are problematic because they can prevent the genetically changed population of an organism from taking over a region.

Omar Akbari, an assistant professor of entomology

Credit: University of California, Riverside

This is due to the presence of drive-resistant alleles in those offspring, which make them immune to the gene drive. (Alleles are alternative forms of genes that determine distinct traits that can be passed from parents to offspring.) The impact of such resistant alleles on the ability of the gene drive to spread and suppress populations has previously been estimated as significant, but hasn’t been well-evaluated.

In work outlined in the bioRxiv article, the researchers used mathematical modeling to determine that resistant alleles will have a major impact on attempts to get rid of a species of mosquito on a scale of a continent such as Africa. In other words, an attempt to eliminate a species of mosquito using this technique would result in a rapid rebound of the suppressed population of mosquitoes due to resistance alleles.

To address this issue, the researchers devised and validated a technique that involves multiplexing guide RNAs within the gene drive. Multiplexing means targeting multiple locations in a gene with the guide RNAs.

Modeling by the research team suggests that the size of the population that can be suppressed increases exponentially with the number of multiplexed guide RNAs. It also shows that with six multiplexed guide RNAs, a mosquito species could potentially be suppressed on a continental scale.

The researchers also demonstrated successful multiplexing in a fruit fly (Drosophila melanogaster), an organism commonly used as a model in labs. Now, they are working to adapt that same technique to mosquitoes.

Contacts and sources:
Sean Nealon

University of California, Riverside


The paper is called, “Overcoming evolved resistance to population-suppressing homing-based gene drives.” The authors are: Omar Akbari, an assistant professor of entomology at UC Riverside; Anna Buchman, a post-doctoral researcher working in Akbari’s lab; John M. Marshall, an assistant professor at UC Berkeley; and Hector Sanchez, a graduate student at the Monterrey Institute of Technology and Higher Education in Mexico.


We encourage you to Share our Reports, Analyses, Breaking News and Videos. Simply Click your Favorite Social Media Button and Share.

Report abuse


Your Comments
Question   Razz  Sad   Evil  Exclaim  Smile  Redface  Biggrin  Surprised  Eek   Confused   Cool  LOL   Mad   Twisted  Rolleyes   Wink  Idea  Arrow  Neutral  Cry   Mr. Green

Top Stories
Recent Stories



Top Global


Top Alternative




Email this story
Email this story

If you really want to ban this commenter, please write down the reason:

If you really want to disable all recommended stories, click on OK button. After that, you will be redirect to your options page.