Novel Technique For Single Protein Observation
Researchers at the Johannes Gutenberg University (JGU) in Mainz, Germany are responsible for the new technique. ‘Our method allows live tracking of individual proteins without having to label them first,’ explains Professor Carsten Sönnichsen of the Institute of Physical Chemistry at JGU, the senior author of the study,. ‘We are now gaining entirely new insights into molecular processes and can see, for example, how things are constantly in motion even on the very smallest scale.’
This innovative method is based on the use of gold nanoparticles, which act as glistening nano-antennas that change their frequency (or colour) when single, unlabelled proteins are detected. ‘This is an enormous leap forward technologically,’ says Professor Sönnichsen. ‘We have managed to achieve a very high time resolution for the observation of individual molecules.’
Researchers can now precisely observe the dynamics of a protein molecule down to the millisecond. This latest development will allow scientists to push forward with research related to proteins, particularly by tracking the fluctuation of protein population densities and by monitoring protein absorption processes in real time.
‘We can see how molecules move, how they dock at particular locations, and how they fold – this has given us a window into the molecular world,’ says Dr Irene Ament, a member of Sönnichsen’s group and the lead author of the study.
This new technique could benefit the fields of chemistry, biology and medicine. Experts say this development is a key element in research into non-equilibrium phenomena at the molecular level.
Contact and sources:
CORDIS
Johannes Gutenberg University Mainz (JGU):
http://www.uni-mainz.de/eng/
Nano Letters:
http://pubs.acs.org/journal/nalefd
European Research Council:
http://erc.europa.eu/
Read more at Nano Patents and Innovations
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