The Stickiness of Underwater Glue of Mussels Made Stickier

“Most synthetic glues do not work on wet surfaces, but mussel foot proteins (Mfp) can adhere to surfaces even underwater,” said Fuzhong Zhang, associate professor of energy, environmental & chemical engineering. “This unique property makes Mfp suitable for many applications, from underwater repair to biomedical glues. With further engineering, an Mfp glue could be used to heal wounds, or maybe even to replace sutures.”

Zhang has received a $502,034 grant from the Office of Naval Research to improve on their initial success, microbially produced underwater adhesives that are stronger than those created naturally by mussels.

Research in the Zhang lab focuses on engineering microbes to produce renewable materials and chemicals with desired properties, a field called synthetic biology. An example of his team’s work includes engineering bacteria that produced synthetic spider silk as strong and tough as natural spider silk fibers. For adhesive proteins, in research published in late 2018, his team demonstrated that synthetic proteins made by engineered bacteria can be even more adhesive than natural Mfps when used underwater.

The power of synthetic biology is that researchers can fine tune the property of protein materials by manipulating their genetic codes. “We can change one parameter each time,” Zhang said. For instance, the length of a chain of Mfp5. “If with an increase of one parameter, we see adhesion becomes better and better, then we can say, ‘OK, this parameter is able to increase adhesion,’” he said.

It turns out, that is exactly what the team has found: when the Mfp protein chain was made longer, its underwater adhesion was stronger than that with natural chain length.

This approach could allow them to explore many unknown mechanisms that control underwater adhesion, parameter-by-parameter.

“Understanding the mechanism by synthesis has the potential to lead to additional enhancements in adhesion, or improve other glue properties.” Zhang said. “In general, we’re trying to understand the mechanism using synthetic biology by producing and testing the materials.”

Contacts and sources:
The McKelvey School of Engineering
Washington University in St. Louis