A Genetic Key for Sperm Production?

Credit: Kyoto University

The scientists injected two types of SSCs into mouse testes: normal cells in some, and Myc gene-suppressed in others. Two months later, they found that the total number of abnormal SSCs was far fewer than normal ones. Gene analysis showed that the capacity for self-renewal had been compromised, with possibly important implications for sperm production in these mice.

“We found changes in the expression of genes that would slow the cell cycle,” says Shinohara.

In other words, suppressed SSCs could self-renew, but at a slower than normal rate. Further study showed that this diminished rate was accompanied by impaired glycolysis, suggesting that the cells were not generating sufficient energy.

“A difference in glycolysis could explain natural differences in SSC self-renewal between mice,” elaborates Mito Kanatsu-Shinohara, first-author of the paper. “DBA/2 and B6 are two mouse types in which SSCs are know to self-renew at different rates.”

Further experiments confirmed that glycolysis was more active in the cells of DBA/2 mice. Moreover, isolating cells from B6 mice and treating them with certain chemicals that enhanced glycolysis could increase the proliferation rate to levels comparable with DBA/2.

“These findings could have important implications for infertility research in the future,” says Shinohara. “Stimulating the metabolism of SSCs could improve their proliferation. However, more careful study of the molecular pathways is necessary.”