More Virulent Malaria Parasites Evolve When Vaccine Is Used Say Penn State Researchers

No malaria vaccine ever has been approved for widespread use. “Effective malaria vaccines are notoriously difficult to develop because the malaria parasite is very complex. Hundreds of different malaria strains exist simultaneously within any local region where the disease is prevalent,” Read said. Most vaccine developers use only small sections of the malaria parasite to produce an antigen molecule that then becomes a key ingredient in a highly purified malaria vaccine. Read’s lab tested the antigen AMA-1, a component of several such vaccines now in various stages of clinical trials.

“Our laboratory experiments followed clues from theoretical studies and earlier experiments that suggested that some malaria vaccines could favor the evolution of more-virulent malaria parasites,” Read said. If candidate vaccines do not completely eliminate all the malaria parasites, the parasites that remain have opportunities to evolve. A mosquito then could transfer the evolved parasite from the vaccinated person into a new host — a process called leaking. “Leaky vaccines create a situation that further fosters parasite evolution,” Read said.

The image shows red blood cells, some of which are infected with ring-stage malaria parasites. Parasites invade, replicate and rupture from red blood cells multiple times during an infection. 

Source:Wikimedia Commons,

The study found that parasites causing worse malaria symptoms in unvaccinated mice evolved after “leaking” consecutively through as few as 10 vaccinated mice. “The parasites that are able to survive in the immunized hosts must be stronger after having survived exposure to the vaccine,” Read said. “The vaccine-induced immunity apparently removed the less virulent malaria parasites, but left the more virulent ones.”

The AMA-1 antigen used in the study triggers the body to make anti-malaria antibodies. These antibodies recognize the AMA-1 antigen on the parasites and disable the malaria infection. The shape of the antigen ensures that the antibodies can bind securely with the malaria parasite — like pieces in a jigsaw puzzle — an important step in producing immunity. Scientists already knew that vaccines become obsolete when evolutionary mutations change the parasite’s antigen structure in such a way that the antibody is not able to lock onto the targeted part of the parasite. But the study showed the malaria parasite evolved within the vaccinated mice even without any detectable changes in the antibody target on the parasite.

“We were surprised to find that more-virulent strains of malaria evolved even while the gene encoding the key antigen remained unchanged,” said Victoria Barclay, the postdoctoral scholar in Read’s lab who conducted the laboratory experiments and who is the corresponding author of the PLoS Biology paper. “We did not detect any changes in the gene sequence.” The researchers conclude that evolution must have taken place somewhere in the parasite’s genome. Read’s lab now is hunting for the exact locations on the parasite’s DNA where the mutations occurred.

The image shows red blood cells, some of which are infected with ring-stage malaria parasites. Parasites invade, replicate and rupture from red blood cells multiple times during an infection. 

Credit: Dr. Mae Melvin/U.S. Centers for Disease Control and Prevention

“Generalizing from animal models is notoriously difficult in malaria,” Read said, so the scientists do not yet know if this newly recognized type of evolution could happen in human malaria or with other rapidly evolving diseases, such as the viruses that cause AIDS or cervical cancer. “What we do know is that in Victoria Barclay’s experiments in our lab at Penn State; with our parasites, our mice, and with this particular antigen; the malaria parasites that evolved through vaccinated hosts become more virulent,” he said.

“Vaccines are one of the most fantastically cost-effective health gains we’ve ever had, so there is no question that we should proceed on all fronts to develop a safe and effective vaccine against malaria,” Read said. “At the same time, our research is revealing new reasons to proceed with vigilant caution.” Read suggests that vaccine researchers conducting clinical trials should not only be carefully monitoring for parasite evolution at the vaccine target, but they also should watch for mutations throughout the parasite’s entire genome. “This sort of monitoring also should go on once a new vaccine goes into widespread use,” he said. “It appears that in a world with leaky vaccines, virulent pathogen strains can evolve. Different vaccines or other transmission-blocking measures might be needed to stop the spread of any evolved parasites,” Read said.

This research received financial support from Penn State.

Contacts and sources:

Barbara Kennedy

Andrew Read

Penn State University

 To watch Read give a TED talk about natural selection as it pertains to the mutation of disease, visit http://www.youtube.com/watch?v=cvXc9aMF6CA.