3D Bioprinted Human Cartilage Cells Can Be Implanted

The researchers printed a hydrogel of nanocellulose mixed with human-derived cartilage cells – a so called construct. They used a 3D bioprinter manufactured by Cellink, a Gothenburg-based startup firm whose bio-ink is a result of research by Paul Gatenholm. Immediately after printing, the construct was implanted in mice.

The researchers can report three positive results of the animal study:
1. Human cartilage tissue has grown in an animal model.
2. Vascularisation, i.e., the formation of blood vessels, between the materials.
3. Strong stimulation of proliferation and neocartilage formation by human stem cells.

“What we see after 60 days is something that begins to resemble cartilage. It is white and the human cartilage cells are alive and producing what they are supposed to. We have also been able to stimulate the cartilage cells by adding stem cells, which clearly promoted further cell division,” says Lars Kölby.

“We now have proof that the 3D printed hydrogel with cells can be implanted. It grows in mice and, in addition, blood vessels have formed in it,” says Paul Gatenholm.

Collaboration has been a key component and critical to the success of the project. Scientists in two different disciplines have successfully crossed academic lines to find a common goal where they could combine their skills in a fruitful way.

“Often, it is like this: we clinicians work with problems and researchers work with solutions. If we can come together, there is a chance of actually solving some of the problems we are wrestling with – and in this way, patients benefit from the research,” says Lars Kölby.

Paul Gatenholm is careful to point out that the results he and Lars Kölby’s team are now able to report do not involve any short cut to bioprinted organs.

“With what we have done, the research has taken a step forward towards someday, we hope, being able to bioprint cells that become body parts for patients. This is how you have to work when it comes to this kind of pioneering activity: one small step at a time. Our results are not a revolution – but they are a gratifying part of an evolution!”

Contacts and sources:
Chalmers University of Technology

Citation: In Vivo Chondrogenesis in 3D Bioprinted Human Cell-laden Hydrogel Constructs
Möller, Thomas; Amoroso, Matteo; Hägg, Daniel; Brantsing, Camilla; Rotter, Nicole; Apelgren, Peter; Lindahl, Anders; Kölby, Lars; Gatenholm, Paul
Plastic and Reconstructive Surgery – Global Open:
February 2017 – Volume 5 – Issue 2 – p e1227
doi: 10.1097/GOX.0000000000001227