Chemists Bring Mixed Folded Proteins to Life
The alumina nanoparticle-assisted enzyme refolding process is shown.
Credit: ITMO University
Proteins, especially including enzymes as accelerators of chemical reactions, are the basis of pharmaceutical and food industries. Meanwhile, 80% of these substances are lost during synthesis. Influenced by unfavorable factors like strong acids, alkalis or heating, proteins denature, that is they lose the native shape and simultaneously any chemical activity. Thus, the industry seeks for a universal method for recovering protein structure, which could make the production cheaper and more effective. To manufacture enzyme-based drugs and foods on an industrial scale it is especially important to find a way to recover mixtures of proteins as renaturating each particular type of enzymes separately takes a lot of time and is costly and inefficient.
Russian chemists in cooperation with foreign colleagues proposed a solution to this issue. They developed a technology process that gave a second life to proteins having returned their molecules to the original form after denaturation.
In the new research, the chemists unfolded molecules of three enzymes: carbonic anhydrase, phosphatase and peroxidase. Denatured by a strong alkaline, the proteins were mixed with nanoparticles of alumina in water. Due to electrostatic interaction, the enzymes attracted the nanoparticles and engaged them in forming a supramolecular complex with not chemical, but physical bonds.
This shell of nanoparticles protected protein molecules from aggregation enabling the scientists to easily extract them from the aggressive media. Washed from denaturing substances, the enzymes restored their structure by themselves.
Changing pH, the scientists separated nanoparticles from proteins showing that the substances involved in the experiment can be repeatedly used.
The authors applied their method to a mixture of two enzymes: carbonic anhydrase and phosphatase (CAB and AcP). For these proteins, the portion of renaturated molecules was more than half, which was an unprecedented result.
Dmitry Malkov
Citation: Katerina V. Volodina, David Avnir and Vladimir V. Vinogradov (2017), Alumina nanoparticle-assisted enzyme refolding: A versatile methodology for proteins renaturation, Scientific Reports, http://www.nature.com/articles/s41598-017-01436-6
Source: http://www.ineffableisland.com/2017/06/chemists-bring-mixed-folded-proteins-to.html
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