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A Step Closer To Biofuel – Algae Carbohydrates Into Lipids

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Kobe University engineers have developed a technique to repartition carbon resources from carbohydrates to lipids in microalgae. They hope that this method can be applied to biofuel production.

A cross-institutional collaboration developed the technique to repartition carbon resources from carbohydrates to lipids in microalgae. This discovery was the result of a collaboration between a research group at Kobe University’s Engineering Biology Research Center consisting of Project Assistant Professor KATO Yuichi and Professor HASUNUMA Tomohisa et al., and Senior Researcher SATOH Katsuya et al. at the Takasaki Advanced Radiation Research Institute of the Quantum Beam Science Research Directorate (National institutes for Quantum and Radiological Science and Technology).

Electron microscope image of lipid production in the microalgae Chlamydomonas. Image Credit: Kobe University. Click image for the largest view.

The research results have been published in the international academic journal Communications Biology.

The press release offered these main points:

  • Microalgae are highly capable of producing lipids by fixing atmospheric CO2 via photosynthesis, making them promising candidates for biofuel production.
  • In light/dark conditions (i.e. day and night), the majority of microalgae’s carbon resources obtained from CO2 are accumulated as carbohydrates (starch). This makes it difficult to get microalgae to produce lipids.
  • The researchers used ion beam mutagenesis to develop a strain of microalgae that can produce large amounts of lipids even under light/dark conditions.
  • In this microalgae mutant, the starch debranching enzyme gene was disrupted, causing it to produce phytoglycogen, which is easily broken down. The carbon resources were then repartitioned from carbohydrate production to lipid production.

Biofuels are renewable resources that have received much attention in the move towards creating more sustainable societies. Microalgae are photosynthetic organisms that are highly capable of producing lipids from carbon dioxide in the atmosphere, making them promising candidates for biofuel production. However, a Kobe University research group consisting of Project Assistant Professor Kato Yuichi and Professor Hasunuma Tomohisa et al. discovered that the majority of carbon resources were diverted to starch production instead of lipid production under light/dark conditions (i.e. day and night). This is a problem when cultivating microalgae species outside.

For this research study, Project Assistant Professor Kato and Professor Hasunuma’s Kobe University research group collaborated with Senior Researcher Satoh et al. at the National institutes for Quantum and Radiological Science and Technology (QST). The researchers used the ion beam at QST’s Takasaki Advanced Radiation Research Institute to induce mutation in the microalgae. This enabled them to cultivate a new mutant strain called Chlamydomonas sp. KOR1 (*1), which can produce large quantities of lipids even in light/dark conditions.

The researchers discovered that this KOR1 strain has disruptions in the starch debranching enzyme (*2) gene ISA1, causing it to produce a different carbohydrate: phytoglycogen (*3) instead of starch.

Normally, microalgae synthesize and accumulate carbohydrates (starch) during light periods and break them down when it is dark. However, many carbohydrates accumulate that cannot be completely broken down. Contrary to this, the carbohydrate synthesized by KOR1 (phytoglycogen) was completely broken down during the dark period. The results of the KOR1 metabolome analysis (*4) revealed a total increase in intermediate metabolites in both the starch and lipid synthesis pathways (intermediate metabolites included fructose-6-phosphate, glucose-6-phosphate, acetyl-CoA and glycerol 3-phosphate).

From this analysis, the researchers illuminated the metabolic mechanism underlying the increased lipid production that resulted from ISA1 gene disruption. In the KOR1 strain, the carbohydrate (phytoglycogen) was quickly broken down and intermediate metabolites subsequently induced the carbon resource to be repartitioned to lipid production.

In order to produce biofuels using microalgae, it is necessary to cultivate these organisms outside in the sunlight. However, there is an unavoidable decrease in lipid production under these light/dark conditions. The technique of “repartitioning carbon resources by disrupting the starch debranching enzyme gene” developed through this research is one answer to this problem. It is hoped that this new method can contribute towards the large-scale implementation of biofuel production using microalgae.

The Kobe University press release provided the following glossary of terms to assist is understanding the text:

  1. Chlamydomonas sp. KOR1: Using the microalgae Chlamydomonas sp. JSC4 (isolated from brackish waters in Taiwan) as the parental strain, KOR1 is a mutant obtained via ion beam mutagenesis (*5). It can be cultivated in either freshwater or saltwater. With its ability to break down carbohydrates and convert them into lipids, it has both a high proliferation rate and a high lipid accumulation rate, demonstrating highly efficient lipid production.
  2. Starch debranching enzyme: Protein that slices through starch’s branch structure. It not only breaks down starch but also creates appropriate structures for synthesis.
  3. Phytoglycogen: Phytoglycogen has a highly branched structure compared to starch. It is also highly soluble in water, which means that it is easily broken down and is easy for intercellular enzymes to use.
  4. Metabolome analysis: A method of comprehensively analyzing ionic small molecules in a sample using CE-TOFMS (Capillary electrophoresis-time of flight mass spectrometry) apparatus.
  5. Ion beam mutagenesis: This involves hitting cells in a plant or microorganism with the accelerated ion molecules of various atoms (such as carbon) at tenths of the speed of light using a particle accelerator. This irradiation technique alters DNA, thus making it possible to create a breed with useful characteristics.

The rarity of Algae press releases has been quite the driver for some disappointment. But here today is a payoff for dedicated algae enthusiasts. And quite good news it is. While carbs are a welcome product, a lipid is far better and much more easily processed into a fuel product. Algae as a biofuel candidate has an excellent foundation of potential, but has had a difficult time getting through to scaleable processing for fuel. This team’s breakthrough is a big step forward.

The post A Step Closer To Biofuel – Algae Carbohydrates Into Lipids first appeared on New Energy and Fuel.


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