To Save Florida's Famous Oranges, Scientists Race to Weaponize a Virus - Wired
With the vector as its syringe, Southern Gardens just needed a drug to inject. Luckily, it had already screened dozens of genes in its search for a resistant tree—from vegetables, viruses, and even a pig. One particularly promising set of genes came from the spinach plant, coding for a group of antibacterial proteins called defensins. (You have defensins too, fighting off microbes in your saliva.) These spinach defensins worked especially well to chop up C. liberibacter, the bacterium that kills citrus trees by choking off their food supplies.
Southern Gardens inserted the spinach genes into the viral vector; all that was left to do was take it to where the bacteria live.
Trees don’t have veins, but they do have a circulatory system of sorts. Called phloem, these pipe-like tissues transport water, sugar, and nutrients from the roots to the leaves and fruit. That’s where C. liberibacter make their home, living off the trees’ food, preventing the fruits from accumulating sugars (that’s why they stay small and green, hence “greening disease”), and eventually starving the tree to death. To access that network, Southern Gardens grafts a vector-treated plant onto the infected tree. As the virus replicates inside the tree, it becomes a spinach defensin factory, and those proteins travel through the phloem attacking every C. literibacter they meet.
“You can think of like a slow release vitamin tablet,” says Tim Eyrich, Southern Gardens’ vice president of research and commercialization. “We’re attacking the bacteria where it lives, and we can do it without changing the biology of the tree at all.” That last bit is really important for the company, which has struggled to convince Americans that GMOs are the only way to keep domestic orange juice on the breakfast table. OJ made from transgenic fruit may carry a GMO label, while juice made from a vector-treated tree would not. And while they’re not giving up hope on a transgenic approach to replace Florida’s lost acres just yet—Southern Gardens is currently testing trees with the same spinach defensins—they’re still a long way from winning regulatory approval, not to mention hearts and minds.
In the meantime though, their weaponized virus is bringing new hope to an increasingly desperate industry. According to economists at the University of Florida, the state’s production has decreased by 70 percent since 2005.,,,
To Save Florida’s Famous Oranges, Scientists Race to Weaponize a Virus | WIRED
Source: http://gmopundit.blogspot.com/2017/04/to-save-floridas-famous-oranges.html
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