Genome Architecture Determines the Fate of a Cell
A new study shows an unexpected and crucial role of genome architecture in determining cell fate.Scientists have long been reading the code of life – the genome –, as a sequence of letters but now researchers have also started exploring its three-dimensional organisation.
In a paper published in Nature Genetics, an interdisciplinary research team of scientists from the Centre for Genomic Regulation (CRG) – including a Centro Nacional de Análisis Genómico (CNAG-CRG) group – in Barcelona, Spain, shows that the three-dimensional organisation of the genome plays a key role in gene expression and consequently in determining cell fate.
It all began with the 4D Genome project, an ambitious and innovative research initiative funded by a European Research Council Synergy grant aimed at understanding how the spatial organisation of the genome contributes to decisions made by cells. What scientists wanted to find out was whether genome architecture plays an active function or whether it is a mere side effect of the genome’s activity.
Understanding how stem cells are formed and how they can convert into different cell types is a major challenge in modern biology. It has been known since the fifties that all specialized cells in the body contain the same genome. So, what then distinguishes one cell type from another? The answer is that different cells read the information contained in different parts of the genome. The machinery required to achieve this selective information recall resides primarily in so called ‘transcription factors’, molecules that switch genes on or off.
“We expected that transcription factors would first switch on certain genes, which then afterwards may force a re-organisation of the 3D structure of the chromosome. Surprisingly, what we found is that in a large proportion of the genome, the transcription factors were actually promoting this re-organisation before the genes were switched on,” explains Ralph Stadhouders, co-first author of the paper together with computational biologist Enrique Vidal.
“Our paper shows that transcription factors may play a completely new role in cell reprogramming: they do not only switch genes on and off, but also promote the architectural changes necessary to modify gene expression,” states Thomas Graf, senior CRG group leader and lead investigator of this study. “It may have relevant implications for researchers worldwide studying gene regulation in general since we know now that the large-scale organization of the genome cannot be ignored. It also raises new questions about how these large changes can be brought about: possibly new mechanisms, perhaps even new machineries that are necessary to change certain areas of the genome” reflects Graf.
The perfect combination: risky + interdisciplinary fundamental science
The findings reported in Nature Genetics indicate that genome architecture has important informational value for controlling gene expression during cell reprogramming, and is thus required for the specialized functions of a cell. “We are only scratching the surface of what could be critical as yet unrecognized mechanisms by which cells regulate gene expression” says co-principal investigator and ICREA research professor at CNAG-CRG, Marc A. Martí-Renom. “The new discovery might also be fundamental for development and for some development-related diseases and cancer,” he concludes.
Again, this is an example on how risky science with innovative and interdisciplinary approaches leads to surprising and relevant results to advance knowledge. In this case, four laboratories at the Centre for Genomic Regulation (including a CNAG-CRG one) took full advantage of their experience and diverse capabilities to address fundamental questions in genome biology with the support of a 13M € ERC Synergy grant*. “Promoting and supporting fundamental research is crucial to advance knowledge. We are proud that our project is already providing the first answers to the burning question about how genome 3D organisation shapes genome regulation,” agree the four group leaders participating in the 4D Genome project: Thomas Graf, Marc A. Martí-Renom, Guillaume Filion, and Miguel Beato.
Contacts and sources:
Laia Cendrós
Centre for Genomic Regulation (CRG)
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