How Fungus Knows Which Way Is Up
It was already known that the fruiting bodies of this fungus sense gravity and grow upright by detecting how octahedral protein crystals in fluid-filled chambers (vacuoles) settle under their own weight. But how the fungus acquired this trick during its evolution has remained unclear. To solve this puzzle, the team purified the crystals and identified a protein which they named OCTIN as their primary building block.
Phycomyces fruiting bodies. Each stalk is a single cell that elongates to form a structure 1-3 cm tall, with a spore-containing sphere at its tip. The spores accumulate melanin as they mature, explaining the black color. Inset: An OCTIN crystal from Phycomyces blakesleeanus; the crystal is about 5 microns across, dwarfing typical bacteria (1-2 microns in length) from which the OCTIN gene is likely to have been acquired.
Credit: Tu Anh Nguyen
“We were surprised that OCTIN-related genes are found in bacteria and that all the evidence pointed to horizontal gene transfer from bacteria into the ancestor of Phycomyces,” said the authors. “This was intriguing because estimates of sedimentation show that bacteria are too small to employ gravity sensing structures. This made it clear that we were looking at the emergence of an evolutionary novelty based on how the proteins assembled.”
In the case of OCTIN, the researchers noticed that the position of cysteine residues, which have the capacity to form molecular bonds within and between proteins, have become rearranged during the evolution of fungal OCTIN. Correspondingly, the fungal OCTIN crystals dramatically swell and dissolve upon the addition of chemical agents that break such bonds, indicating that they indeed play a critical role in holding the crystal lattice together.
The authors note that OCTIN itself is not the end of the story; when they forced mammalian cell to make fungal OCTIN, it did not form crystals. This suggests that the fungus has co-factors that are required to assist crystal assembly. Dr Jedd adds, “We are currently searching for these factors with the aim of reconstituting OCTIN crystal formation in the test tube. This will allow us to better understand and manipulate the assembly process and its products.
Contacts and sources:
Gregory Jedd
PLOS
Source: http://www.ineffableisland.com/2018/04/how-fungus-knows-which-way-is-up.html
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