Neandertal Genes Influence Brain Development of Modern Humans

Endocasts of fossil and modern skulls

The authors combine paleoanthropology, archaic genomics, neuroimaging and gene expression to study biological foundations of the characteristic modern human endocranial shape. Shown here are the coordinate measurements used to capture endocranial shape from magnetic resonance images of several thousand modern humans.

© © CC BY-NC-ND 4.0 (Image: Philipp Gunz)

The researchers first used computed tomographic scans of fossil Neandertal skulls and skulls of modern humans to make endocasts – virtual imprints of the interior of the braincase. They then developed a single measure of globularity, based on the differences in skull shape between humans and Neandertals. Next, the scientists teamed up with colleagues at the Radboud University, the University of Greifswald and UC Irvine, to determine the degree of globularity of thousands of healthy present-day humans, using data from magnetic resonance imaging. Although modern human brain and skull shapes are all clearly distinct from those of Neandertals, the scientists still found considerable differences in globularity among the participants. Finally, the researchers studied the genomes of around 4,500 of the participants to identify the fragments of Neandertal DNA that each person carried. Would any of these Neandertal DNA fragments influence brain globularity in their living human sample?

The team found Neandertal DNA fragments on chromosomes 1 and 18 that were associated with less globular (more elongated) brains. These fragments were associated with altered activity of two genes, UBR4 and PHLPP1, which are already known to play roles in important aspects of brain development (neurogenesis and myelination respectively). The strongest evidence for effects of these Neandertal DNA fragments on gene activity were in the putamen (in the basal ganglia) and the cerebellum. “The potential for links between evolutionary changes in brain globularity and mechanisms affecting the basal ganglia and cerebellum is intriguing”, says Gunz. Both structures receive direct input from the motor cortex and are involved in the preparation, learning, and coordination of movements. The basal ganglia also contribute to cognitive functions such as memory, attention, planning, skill learning, and potentially speech and language evolution.
No conclusions on cognitive skills

Computed tomographicscan of the Neandertal fossil from La Chapelle-aux-Saints (left) with a typical elongated endocranial imprint (red) and of a modern human (right) showing the characteristic globular endocranial shape (blue). Arrows highlight the enlarged posterior cranial fossa (housing the cerebellum) as well as bulging of parietal bones in modern humans compared to Neandertals.[less]

© © CC BY-NC-ND 4.0 (Image: Philipp Gunz)

The authors stress that recent archaeological evidence has documented sophisticated symbolic behaviours in Neandertals that had previously been attributed exclusively to modern humans, such as the enigmatic structure built deep inside Bruniquel cave, and Neandertal cave-art from Iberia. As Gunz notes, “The focus of our study is on understanding the unusual brain shape of modern humans. These results cannot be used to make inferences about what Neandertals could or could not do”.

“The effects of carrying these rare Neandertal DNA fragments are really subtle, but detectable due to the large sample size”, explains Fisher, adding “This is only our first glimpse of the molecular underpinnings of globularity. Like other aspects of brain structure, globularity is a trait that is likely to be influenced by the combined effects of many different genetic variants.”

According to the research team, this discovery generates hypotheses that can be tested with new experiments, for example using human neuronal tissue that can be grown in the laboratory. Gunz and Fisher are now scaling-up the approach for investigations in larger samples such as the UK Biobank. They anticipate that future genome-wide screening studies will reveal additional genes associated with globularity, as well as indicate how this fascinating trait is linked to other aspects of human biology.

Contacts and sources:
Marjolein Scherphuis
Max Planck Institute for Psycholinguistics

Sandra Jacob
Max Planck Institute for Evolutionary Anthropology

Citation: Neandertal introgression sheds light on modern human endocranial globularity.
Philipp Gunz, Amanda K. Tilot, Katharina Wittfeld, Alexander Teumer, Chin Yang Shapland, Theo G.M. van Erp, Michael Dannemann, Benjamin Vernot, Simon Neubauer, Tulio Guadalupe, Guillen Fernández, Han G. Brunner, Wolfgang Enard, James Fallon, Norbert Hosten, Uwe Völker, Antonio Profico, Fabio Di Vincenzo, Giorgio Manzi, Janet Kelso, Beate St. Pourcain, Jean-Jacques Hublin, Barbara Franke, Svante Pääbo, Fabio Macciardi, Hans J. Grabe, and Simon E. Fisher
Current Biology, 13 December 2018, DOI: 10.1016/j.cub.2018.10.065